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Depth of invasion is a newly added index for TNM staging of oral cancer in the eighth edition. Preoperative evaluation of depth of invasion not only provides a reference for surgical margin, but also serves as an independent prognostic factor for predicting lymph node metastases. At present, the main methods for assessing the depth of invasion of tongue squamous cell carcinoma include ultrasound examination, MRI, CT, positron emission tomography (PET) and histopathological examination. This paper summarizes the evaluation method and clinical effect of depth of invasion of tongue cancer, and analyzes its advantages and boundedness. In addition, this study is expected to provide a reference for the surgical treatment of tongue squamous cell carcinoma.
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Objective:To investigate the association of the rsl801133 polymorphisms of the methylenetetrahydrofolate reductase (MTHFR)gene and rs2236225 polymorphisms of the methylenetetrahydrofolate dehydrogenase(MTHFD1)gene with non-syndromic cleft lip with or without cleft palate (NSCL/P)in Chinese population of Shanxi Province.Methods:The rsl801133 polymorphism of MTHFR gene and rs2236225 polymorphism of MTHFD1 gene were examined by PCR-RFLP in 265 patients with NSCL/P and 276 healthy controls.Data were statistically analysed.Results:The genotypic distribution of rsl801133 and rs2236225 was not deviated from the Hardy-Weinberg equilibrium.There was no significant difference in allele frequencies of rsl801133 and rs2236225 variants between patients with NSCL/P and healthy individuals(P <0.05).Conclusion:The polymorphism of MTHFR gene and MTHFD1 gene was not associated with NSCL/P in Chinese population of Shanxi Province.
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<p><b>OBJECTIVE</b>To assess the association between polymorphism of methylenetetrahydrofolate reductase (MTHFR) gene C677T locus and nonsyndromic cleft lip with or without cleft palate (NSCL/P) in Shanxi Province of China.</p><p><b>METHODS</b>Blood samples from 150 patients and their parents and 150 controls and their mothers were collected. The polymorphism of MTHFR gene C677T locus were analyzed by the methods of polymerase chain reaction and restriction fragment length polymorphism technique(PCR-RFLP). Case-control analysis, transmission-disequilibrium test(TDT) and haplotype-based haplotype relative risk analysis(HHRR) were used to study the correlation between the gene mutation and NSCL/P.</p><p><b>RESULTS</b>Hardy-Weinberg equilibrium test results showed that, the offspring and mother genotype in the case group and the control group was not deviated from the Hardy-Weinberg law of genetic equilibrium (P > 0.05). The distribution of genotype CC, CT and TT in offspring had significant difference between the two groups (P < 0.05). In the offspring and mother of case group and the control group, the carriers of the TT genotype compared to CC genotype, the OR are greater than 1 and 95% CI do not contain 1 (offspring OR: 2.692, 95% CI: 1.319-5.495; mother OR: 2.469, 95% CI: 1.136-5.363). The distribution of C allele and T mutation gene were significantly different in the offspring and mother between the two groups (P < 0.05). The TDT test showed: chi2 = 4.507, P < 0.05. The HHRR test showed: P < 0.05.</p><p><b>CONCLUSION</b>The single nucleotide polymorphism of MTHFR C677T locus is associated with the development of NSCL/P in Shanxi Province.</p>
Subject(s)
Child , Child, Preschool , Female , Humans , Infant , Male , China , Cleft Lip , Genetics , Cleft Palate , Genetics , Methylenetetrahydrofolate Reductase (NADPH2) , Genetics , Polymorphism, Single NucleotideABSTRACT
Objective To study biological significance of CD44v6 and p63 protein expression in buccal squamous cell carcinomas (BSCC). Methods Immunohistochemical method was used to examine the expression of CD44v6 and p63 protein in 52 cases of BSCC,10 cases of atypical hyperplasia and 10 cases of normal buccal mucosa,which were analyzed based on clinic and pathology characteristics.Results In normal tissue,atypical hyperplastic tissue of buccal mucosa and BSCC tissue,the expression of CD44v6 decreased gradually 90.0 ％ (9/10),80.0 ％ (8/10) and 55.8 ％ (29/52),respectively,and the layers of positive expression was decreased,and the expression of p63 increased gradually,30.0 ％ (3/10),40.0 ％ (4/10) and 90.4 ％(47/52),respectively.The differences of their expression rates were significant (P ＜0.05).The expression of CD44v6 and p63 had no relationship with gender,age,tumor classification,pathological differentiation (P ＞0.05).CD44v6 was relevant with invasion style and lymphatic node metastasis of BSCC (P ＜ 0.05),while p63 has no relevance with lymphatic node metastasis of BSCC (P ＞0.05).Conclusion CD44v6 and p63 participate in the regulation of generation and deve-lopment process of BSCC.CD44v6 can be utilized as a useful marker of BSCC for detection and prediction of prognosis.p63 play a role as oncogene,which can be a useful marker for BSCC detection.CD44v6 and p63 have independent function in generation,development,metastasis and aversion of BSCC.